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In statistics, a result is called statistically significant if it is unlikely to have occurred by chance. "A statistically significant difference" simply means there is statistical evidence that there is a difference; it does not mean the difference is necessarily large, important, or significant in the common meaning of the word.

The significance level of a test is a traditional frequentist statistical hypothesis testing concept. In simple cases, it is defined as the probability of making a decision to reject the null hypothesis when the null hypothesis is actually true (a decision known as a Type I error, or "false positive determination"). The decision is often made using the p-value: if the p-value is less than the significance level, then the null hypothesis is rejected. The smaller the p-value, the more significant the result is said to be.

In more complicated, but practically important cases, the significance level of a test is a probability such that the probablility of making a decision to reject the null hypothesis when the null hypothesis is actually true is no more than the stated probability. This allows for those applications where the probability of deciding to reject may be much smaller than the significance level for some sets of assumptions encompassed within the null hypothesis.

Use in practice[]

The significance level is usually represented by the Greek symbol, α (alpha). Popular levels of significance are 5%, 1% and 0.1%. If a test of significance gives a p-value lower than the α-level, the null hypothesis is rejected. Such results are informally referred to as 'statistically significant'. For example, if someone argues that "there's only one chance in a thousand this could have happened by coincidence," a 0.1% level of statistical significance is being implied. The lower the significance level, the stronger the evidence.

In some situations it is convenient to express the statistical significance as 1 − α. In general, when interpreting a stated significance, one must be careful to note what, precisely, is being tested statistically.

Different α-levels have different advantages and disadvantages. Smaller α-levels give greater confidence in the determination of significance, but run greater risks of failing to reject a false null hypothesis (a Type II error, or "false negative determination"), and so have less statistical power. The selection of an α-level inevitably involves a compromise between significance and power, and consequently between the Type I error and the Type II error.

In some fields, for example nuclear and particle physics, it is common to express statistical significance in units of "σ" (sigma), the standard deviation of a Gaussian distribution. A statistical significance of "" can be converted into a value of α via use of the error function:

The use of σ is motivated by the ubiquitous emergence of the Gaussian distribution in measurement uncertainties. For example, if a theory predicts a parameter to have a value of, say, 100, and one measures the parameter to be 109 ± 3, then one might report the measurement as a "3σ deviation" from the theoretical prediction. In terms of α, this statement is equivalent to saying that "assuming the theory is true, the likelihood of obtaining the experimental result by coincidence is 0.27%" (since 1 − erf(3/√2) = 0.0027).

Fixed significance levels such as those mentioned above may be regarded as useful in exploratory data analyses. However, modern statistical advice is that, where the outcome of a test is essentially the final outcome of an experiment or other study, the p-value should be quoted explicitly. And, importantly, it should be quoted whether or not the p-value is judged to be significant. This is to allow maximum information to be transferred from a summary of the study into meta-analyses.


A common misconception is that a statistically significant result is always of practical significance, or demonstrates a large effect in the population. Unfortunately, this problem is commonly encountered in scientific writing.[1] Given a sufficiently large sample, extremely small and non-notable differences can be found to be statistically significant, and statistical significance says nothing about the practical significance of a difference.

One of the more common problems in significance testing is the tendency for multiple comparisons to yield spurious significant differences even where the null hypothesis is true. For instance, in a study of twenty comparisons, using an α-level of 5%, one comparison will likely yield a significant result despite the null hypothesis being true. In these cases p-values are adjusted in order to control either the false discovery rate or the familywise error rate.

An additional problem is that frequentist analyses of p-values are considered by some to overstate "statistical significance".[2][3] See Bayes factor for details.

Yet another common pitfall often happens when a researcher writes the ambiguous statement "we found no statistically significant difference," which is then misquoted by others as "they found that there was no difference." Actually, statistics cannot be used to prove that there is exactly zero difference between two populations. Failing to find evidence that there is a difference does not constitute evidence that there is no difference. This principle is sometimes described by the maxim "Absence of evidence is not evidence of absence."

According to J. Scott Armstrong, attempts to educate researchers on how to avoid pitfalls of using statistical significance have had little success. In the papers "Significance Tests Harm Progress in Forecasting,"[4] and "Statistical Significance Tests are Unnecessary Even When Properly Done,"[5] Armstrong makes the case that even when done properly, statistical significance tests are of no value. A number of attempts failed to find empirical evidence supporting the use of significance tests. Tests of statistical significance are harmful to the development of scientific knowledge because they distract researchers from the use of proper methods. Armstrong suggests authors should avoid tests of statistical significance; instead, they should report on effect sizes, confidence intervals, replications/extensions, and meta-analyses.

Use of the statistical significance test has been called seriously flawed and unscientific by authors Deirdre McCloskey and Stephen Ziliak. They point out that "insignificance" does not mean unimportant, and propose that the scientific community should abandon usage of the test altogether, as it can cause false hypotheses to be accepted and true hypotheses to be rejected.[6][1]

Signal–noise ratio conceptualisation of significance[]

Statistical significance can be considered to be the confidence one has in a given result. In a comparison study, it is dependent on the relative difference between the groups compared, the amount of measurement and the noise associated with the measurement. In other words, the confidence one has in a given result being non-random (i.e. it is not a consequence of chance) depends on the signal-to-noise ratio (SNR) and the sample size.

Expressed mathematically, the confidence that a result is not by random chance is given by the following formula by Sackett:[7]

For clarity, the above formula is presented in tabular form below.

Dependence of confidence with noise, signal and sample size (tabular form)

Parameter Parameter increases Parameter decreases
Noise Confidence decreases Confidence increases
Signal Confidence increases Confidence decreases
Sample size Confidence increases Confidence decreases

In words, the dependence of confidence is high if the noise is low and/or the sample size is large and/or the effect size (signal) is large. The confidence of a result (and its associated confidence interval) is not dependent on effect size alone. If the sample size is large and the noise is low a small effect size can be measured with great confidence. Whether a small effect size is considered important is dependent on the context of the events compared.

In medicine, small effect sizes (reflected by small increases of risk) are often considered clinically relevant and are frequently used to guide treatment decisions (if there is great confidence in them). Whether a given treatment is considered a worthy endeavour is dependent on the risks, benefits and costs.

Tests of statistical significance[]


See also[]


  1. 1.0 1.1 Ziliak, Stephen T. and Deirde N. McCloskey. "Size Matters: The Standard Error of Regressions in the American Economic Review" (August 2004). [1]
  2. Goodman S (1999). Toward evidence-based medical statistics. 1: The P value fallacy.. Ann Intern Med 130 (12): 995–1004.
  3. Goodman S (1999). Toward evidence-based medical statistics. 2: The Bayes factor.. Ann Intern Med 130 (12): 1005–13.
  4. Armstrong, J. Scott (2007). Significance tests harm progress in forecasting. International Journal of Forecasting 23: 321–327.
  5. Armstrong, J. Scott (2007). Statistical Significance Tests are Unnecessary Even When Properly Done. International Journal of Forecasting 23: 335–336.
  6. McCloskey, Deirdre N.; Stephen T. Ziliak (2008). The Cult of Statistical Significance: How the Standard Error Costs Us Jobs, Justice, and Lives (Economics, Cognition, and Society), The University of Michigan Press.
  7. Sackett DL (October 2001). Why randomized controlled trials fail but needn't: 2. Failure to employ physiological statistics, or the only formula a clinician-trialist is ever likely to need (or understand!). CMAJ 165 (9): 1226–37.

External links[]

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